ETF1 Antikörper

Produktnummer ABIN2720568

Der Maus Monoklonal Anti-ETF1 Antikörper wurde für WB und FACS validiert. Er ist geeignet, ETF1 in Proben von Human zu detektieren.

Kurzübersicht für ETF1 Antikörper (ABIN2720568)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ETF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 2E3

Produktdetails anti-ETF1 Antikörper

Produktmerkmale: Homo sapiens eukaryotic translation termination factor 1 (ETF1)

Aufreinigung: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB 1:500, FLOW 1:100,

Kommentare:

The concentration of the product may vary between diferrent lots.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Andere Bezeichnung: ETF1

Hintergrund: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).

Molekulargewicht: 48.9 kDa

Gen-ID: 2107

NCBI Accession: NM_004730

HGNC: 2107